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Representative Jβ2.7 cleavage gels. Lanes 1 to 6 show a cleavage time course of a radiolabeled Jβ2.7 RSS substrate with no partner RSS, while lanes 8 to 14 show cleavage in purified Jβ2.7-Vβ14 PCs. Lane 8 shows the nonbiotinylated partner control. Lane 7 shows an input lane, representing 33% of the total radiolabeled Jβ2.7 used in binding reactions. Percentages of input radioactivity pulled down (% input), hairpin product (% H), and nicked product (% N) are indicated.
