FIG 1.

The CD4 SP subset in the thymus and CD4⁺ and CD8⁺ T-cell subpopulations in the spleen of Lck-Cre/Fbw7^flox/flox mice are reduced. (A) Representative flow cytometric analysis of surface expression of CD4 and CD8 on thymocytes from Fbw7^flox/flox or Lck-Cre/Fbw7^flox/flox mice at 8 weeks of age. The percentages of DN, DP, and SP populations are indicated. (B) Proportions of the T-cell subsets determined in panel A. Data are means ± SD from six mice of each genotype. (C) Representative flow cytometric analysis of surface expression of CD4 or CD8 on splenocytes from Fbw7^flox/flox or Lck-Cre/Fbw7^flox/flox mice at 9 to 10 weeks of age. The percentages of CD4⁺ and CD8⁺ T-cell subpopulations are indicated. (D) Proportions of the T-cell subsets determined in panel C. Data are means ± SD from six mice of each genotype. The P value was determined using the Student t test. F/F, flox/flox.