(A) Proportions of determinate and indeterminate QuantiFERON-TB Gold In-Tube assay results in children and adolescents (<18 years of age), adults (18 to 64 years of age), and elderly patients (≥65 years of age) (n = 3,263). Mann Whitney U test P values are shown above the bars. (B) Interferon gamma concentrations in the positive-control samples according to age (n = 3,263). The nonlinear regression line is indicated in red. (C) Correlation between age and interferon gamma concentrations in the positive-control samples in the group of children and adolescents (n = 263). The dotted lines indicate the 95% confidence interval. (D) Correlation between age and interferon gamma concentrations in the positive-control samples for adult and elderly patients (n = 3,000). The dotted lines indicate the 95% confidence interval.