TABLE 2.
Sensitivities of different capture ELISAs tested
| Capture MAb (isotype) | Detection MAb | Sensitivity (ng/ml)a |
|---|---|---|
| 5G2 (IgG1) | 4G3 | 333 |
| 4H3 | ND | |
| 6G1 (IgG1) | 4G3 | ND |
| 4H3 | ND | |
| 9H2 (IgG1) | 4G3 | ND |
| 4H3 | ND | |
| 10C12 (IgG1) | 4G3 | 0.5 |
| 4H3 | 0.5 | |
| 4G3 (IgG2b) | 10C12 | 0.5 |
| 5G2 | 37 | |
| 6G1 | ND | |
| 9H2 | ND | |
| 4H3 (IgG2b) | 10C12 | 0.5 |
| 5G2 | ND | |
| 6G1 | ND | |
| 9H2 | ND |
a
Sensitivity is defined by the lowest concentration of SEl-K from which the starting concentration (1 μg/ml) could be calculated by using the linear-fit standard curve generated in each ELISA. Antibody combinations without a sensitivity value listed did not successfully bind SEl-K in this assay; hence, calculations were not done (ND).