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(A) Small membrane filtration (SMF) manifold (version 5.0) evaluated. The SMF manifold device consists of a cylindrical manifold connected to a single vacuum and uses separate control valves at each station for independent operation. (B) The prototype manifold shown contains 10 workstations, each fitted with a stainless steel screw-on port that holds an SMF filter apparatus. (C) Sputum processing and aliquoting protocol.
