FIG 1.

Reproducibility of the MG190 TaqMan PCR assay. (A) M. genitalium G37 organisms were spiked into ThinPrep PreservCyt specimens at concentrations ranging from 1 × 10⁴ to 3 × 10⁷ organisms per ml. DNA was purified from each spiked specimen and used as the template in 10 replicate PCRs run on separate plates. (B) In a separate study, after spiking M. genitalium G37 organisms at the indicated concentrations, five independent DNA purifications and PCRs were performed once weekly over a 5-week period to evaluate interassay precision of the assay system. Data are presented as cycle of quantification (Cq) with percent coefficient of variation for each group.