FIG 3.

Nucleotide alignment of M. genitalium genotypes. Using a previously described genotyping system (15), we PCR amplified and sequenced the specific genotyping region of MG191 from all specimens confirmed to be positive for M. genitalium infection (n = 7). Sequencing reads were trimmed using Sequencher version 4.10.1 and aligned using Clustal W in MacVector version 12.0.3. Sequences were compared to those for previously described M. genitalium strains using NCBI's BLASTn program. M. genitalium strain G37 was processed in parallel to confirm accuracy of the system and is presented as a reference. Nucleotide reference numbers are relative to that for G37 (GenBank accession number NC_000908).