TABLE 1.

Oligonucleotides used in this study

Oligonucleotide	Sequence (5′ to 3′)	Use(s)
stx1-seq-F1a	ATGTCATTCGCTCTGCAATAGGTAC	Amplification and partial sequencing
stx1–3′-R1	TATTTAACATTGATGAACCYCC	Amplification and partial sequencing
stx1–5′-F1	ATGGTGCTCAAGGAGTATTG	Amplification and partial sequencing
stx1-seq-R1a	GAAGAAGAGACTGAAGATTCCATCTG	Amplification and partial sequencing
stx1InvF1	GGCATTCAGCGAATCCACATAC	Inverse nested PCR
stx1InvR1	ACCAGATGGAATCTTCAGTCACTTC	Inverse nested PCR
stx1InvF2	GCTGTGGAGAAATCGACTGTAAAATC	Inverse nested PCR
stx1InvR2	CAAATTACGGGGATGACGATAACC	Inverse nested PCR
stx1e-seq-F1	GCAACCATGCTCTGGAATAC	Amplification of stx1e holotoxin
stx1e-seq-R1	GTGTTGAAACAGAAACGCCT	Amplification of stx1e holotoxin
stx1e-seq-F2	CTAATGCAGTCGCCCAGGAT	stx1e sequencing
stx1e-seq-F3	TTTGTTACGGTGACAGCCGA	stx1e sequencing
stx1e-seq-F4	GTGTTGGCAGCACCTGATTG	stx1e sequencing
stx1e-seq-R2	CCTCACTGAACCCCTCTCCA	stx1e sequencing
stx1e-seq-R3	CTCAGCCTTCCCCAGTTCAG	stx1e sequencing
stx1e-seq-R4	AAAATCCTGGGCGACTGCAT	stx1e sequencing
EcloacStx1F	GGAAGGCTGAGCAGTGTTCTG	stx1e real-time PCR
EcloacStx1R	GGATTGCATTAACCCCTCCAAAAG	stx1e real-time PCR
EcloacStx1TMP	FAM-CCCAACACGAACAGAGTCTTGCCCA-BHQ1b	stx1e real-time PCR

^aPreviously described oligonucleotide (7).

^bFAM, 6-carboxyfluorescein; BHQ1, Black Hole quencher.