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. 2014 Jul;88(14):7764–7775. doi: 10.1128/JVI.00748-14

FIG 2.

FIG 2

Immunization of MHC-II KO mice does not induce vaccine-specific IgG antibodies. (A) Total IgG and IgM antibodies specific for inactivated influenza (A/PR8) virus antigen on day 18 post-prime and day 10 post-boost immunization. (B) Isotype-switched IgG antibodies specific for inactivated influenza (A/PR8) virus antigen after boost immunization. A/PR8 inactivated virus was used as an ELISA plate-coating antigen (400 ng/100 μl/well). (C) Serum antibody levels in mice after PR8 VLP vaccination. Groups of mice (n = 6) were intramuscularly immunized with 3 μg of influenza PR8 VLP vaccine at weeks 0 and 4. At 4 weeks after boost immunization, A/PR8 virus-specific antibody levels were measured (ng/ml). Relative antibody concentrations were determined using a standard measurement of mouse purified IgG, IgM, and each isotype specific to A/PR8 virus. Naive, unimmunized mice B6 WT mice; WT, B6 WT mice (n = 9); KO, MHC-II KO mice (n = 9); CD4KO, CD4 knockout mice. nd, not detectable or similar to naive controls; ns, not significant; **, P < 0.01; ***, P < 0.001.