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. 2014 Jul;88(14):8116–8128. doi: 10.1128/JVI.01014-14

FIG 6.

FIG 6

Glycosylation of CRT is needed for viral assembly. (A) The effect of Tunicamycin (TUN) treatment (0.5 μg/ml) of HPT cells on CRT mRNA expression was analyzed by qPCR. (B) The effect of TUN treatment (0.5 μg/ml) of HPT cells on CRT protein level and CRT size was analyzed by Western blotting using a CRT antibody. (C) The effect of TUN treatment (0.5 μg/ml) of HPT cells on VP28 mRNA expression was analyzed by qPCR. (D) The effect of TUN treatment (0.5 μg/ml) of HPT cells on VP 28 and CRT protein level as detected by Western blotting at 24 h.p.i. The level of actin was analyzed as a control. E and F) The effect of TUN treatment as detailed for panel A on viral structural protein oligomerization in HPT cells analyzed by Western blotting using VP26 antibody (E) or VP28 antibody (F). Significant differences are indicated by asterisks: *, P < 0.05; **, P < 0.01.