FIG 6.

PK digestion of prion-exposed cytosolic VRQ fly head homogenate. Fly head homogenates were prepared from ovine VRQ(cyt) PrP transgenic Drosophila crossed with the Elav-GAL4 driver fly line following exposure at the larval stage to VRQ/VRQ scrapie-free (tracks 1 to 3) or scrapie-infected (tracks 4 to 6) sheep brain homogenate. Samples were incubated with 0, 10, or 30 μg/ml PK at 37°C for 15 min, and the reaction products were analyzed by SDS-PAGE and Western blot analysis with anti-PrP monoclonal antibody Sha31. The equivalent of 10 fly heads was loaded per track. Molecular mass marker values (kDa) are shown on the left of each gel. Ages of flies (in days) are shown on the right.