TABLE 5.
Differences in cellular transcription between seminal CMV shedders and non-shedders
| Characteristica | Valueb |
Univariate P valuec | |
|---|---|---|---|
| Any CMV (n = 15) | No CMV (n = 27) | ||
| HIV RNA copies (us)/ng RNA/CD4 [median (IQR)] | 0.24 (0.09–0.83) | 0.14 (0.08–0.32) | 0.247 |
| HIV RNA copies (ms)/ng RNA/CD4 [median (IQR)] | 0.024 (0.0067–0.103) | 0.003 (0.0–0.03) | 0.017 |
| 2-LTR (copies/106 CD4+ T cells) [median (IQR)] | 0 (0–29.6) | 0 (0–19.7) | 0.983 |
| Any detectable (us) HIV RNA [n (%)] | 15 (100) | 25 (92.6) | 0.530 |
| Any detectable (ms) HIV RNA [n (%)] | 12 (80) | 15 (55.6) | 0.180 |
| Any detectable 2-LTR [n (%)] (total, 50) | 8 (38.1) | 13 (44.8) | 1.000 |
| HIV RNA (us)/HIV DNA ratio [median (IQR)] | 2.17 (1.16–5.63) | 1.57 (0.71–3.13) | 0.183 |
| HIV RNA (ms)/HIV DNA ratio [median (IQR)] | 0.31 (0.01–0.63) | 0 (0–0.42) | 0.068 |
a
Levels of unspliced (us) cellular HIV RNA normalized per ng CD4 T cells, levels of multiply spliced (ms) cellular HIV RNA encoding Tat-Rev normalized per ng CD4 T cells, and total log10 levels of 2-LTR normalized per million CD4+ T cells. The HIV RNA/HIV DNA ratio is the average number of HIV RNA transcripts (i.e., transcriptional activity) per HIV DNA copy. Cellular HIV RNA data were available for a subset of 42 subjects with undetectable HIV RNA levels in blood (<50 copies/ml).
b
Patients with HIV blips in blood were excluded from the analysis.
c
Univariate P values were determined using the Mann-Whitney Wilcoxon test; P values of <0.1 are in boldface.