FIG 7.

Immune cell subpopulations in WT and importin-α7^−/− mice upon influenza virus infection. WT (black bars) or α7^−/− (red bars) mice were infected with 10⁶ PFU of PR8:NS1-GFP (n = 6). Immune cell populations in lung homogenates (A to E) or BAL fluid (F to E) were analyzed at day 3 p.i. with a BD LSR II flow cytometer. Cell frequencies were determined within the total leukocytes (CD45⁺) (graphs with black and red bars) and within GFP⁺ live cells (graphs with green bars). Frequencies of granulocytes (CD11b⁺, CD11c⁻, F4/80⁻, Gr-1⁺) (A and F), macrophages (CD11b⁺, CD11c⁻, F4/80⁺, Gr-1⁺) (B and G), alveolar macrophages (CD11b⁻, CD11c^high) (C and H), and DCs (CD11b⁺, CD11c^high) (D and I) were determined. For a description of the gating strategy used, see Fig. 10. Cell numbers from PBS-treated mice are shown in gray columns. Representative dot plots of GFP⁺ cells are shown to the right of the corresponding graphs. (E and J) Representative plots of the flow-cytometric analysis. *, P < 0.05; **, P < 0.01.