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. 2014 Jul;88(14):7929–7940. doi: 10.1128/JVI.00787-14

FIG 9.

FIG 9

miR-181c directly binds to the HCV genotype 2a genome. (A) Rep2a-Rluc cell lysates were immunoprecipitated with Ago2-specific monoclonal antibody or an unrelated IgG2a isotype control. RNA was isolated from immunoprecipitates by using an RNeasy kit, and HCV RNA expression was analyzed by qRT-PCR. (B) HCV E1 or NS5A was amplified from immunoprecipitates by RT-PCR and analyzed by agarose gel electrophoresis. A representative sample of triplicates is shown. (C) Relative expression levels of miR-181c from Ago2 immunoprecipitates analyzed by qRT-PCR. (D) miR-181c-expressing HCV genotype 2a- or HCV genotype 1a-harboring Huh7.5 cell lysates were immunoprecipitated with Ago2-specific monoclonal antibody. RNA was isolated from immunoprecipitates, and HCV RNA expression was analyzed by qRT-PCR.