TABLE 3.
HSK severity and corneal blink reflex in CD4-depleted micea
| HSK categoryb | Blink reflex status (%) by dpic |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7 |
15 |
20 |
28 |
40 |
60 |
70 |
||||||||
| BR+ | BR− | BR+ | BR− | BR+ | BR− | BR+ | BR− | BR+ | BR− | BR+ | BR− | BR+ | BR− | |
| No opacity | 33 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Mild | 44 | 0 | 0 | 12.5 | 0 | 12.5 | 0 | 12.5 | 12.5 | 12.5 | 12.5 | 25 | 37.5 | 0 |
| Moderate | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 12.5 | 12.5 | 25 | 25 | 12.5 | 12.5 | 25 |
| Severe | 0 | 0 | 0 | 87.5 | 25 | 62.5 | 25 | 50 | 0 | 37.5 | 0 | 25 | 12.5 | 12.5 |
| Total | 88 | 12 | 0 | 100 | 25 | 75 | 25 | 75 | 25 | 75 | 37.5 | 62.5 | 62.5 | 37.5 |
a
Mice were systemically depleted of CD4+ T cells as described in Materials and Methods. Progression to moderate or severe HSK was significantly (P < 0.005) lower in CD4-depleted mice than in mock-depleted mice (Table 2) at 60 and 70 dpi by a chi-square test.
b
Categories of HSK were based on the degree of opacity on HSV-1-infected mouse corneas.
c
Days after corneal HSV-1 infection. Corneas were categorized as blink reflex positive (BR+) or negative (BR−) based on the ability of the mouse to blink when any area of their cornea was touched with a cotton-tipped surgical spear.