TABLE 1.

PCR primer combinations used for amplification of the full protein-encoding region in PLVA and PLVB isolates^a

PCR	Round	PLVA			PLVB
		Forward primer	Reverse primer	Product length (kb)	Forward primer	Reverse primer	Product length
1	1	A3F	A16R	4.2	B3F	P2R	3.3 kb
	2a	A4F	A12R	2.2	B4F	B9R	500 bp
	2b	A9F	A15R	2.4	B7F	P1R	2.5 kb
2	1	P1F	A26R	5.7	B14F	B39R	6.2 kb
	2a	A14F	A21R	3.7	B15F	B25R	2.6 kb
	2b	A19F	A24R	2.1	B24F	B30R	2.0 kb
	2c				B29F	B38R	2.0 kb
3	1	A21F	A32R	2.3	B30F	B44R	2.7 kb
	2	A22F	A31R	1.6	B34F	B43R	1.1 kb

^aTable SA2 in the supplemental material provides the sequence and genome location for each primer.