TABLE 1.
Primers used in this study
| Method and primer | Sequence (5′–3′) |
|---|---|
| RT-qPCR | |
| Env1-S | CATTGGGAAAGGACTTGAT |
| Env1-R | CCCCTTCCTCAGCCAGTAG |
| Env2-S | CTCGCCACACCAGACAGC |
| Env2-R | GCAGCCATGAGCATATCTAGC |
| Env3-S | CTCTTGTCTCCCCCAGTGTG |
| Env3-R | AGTCTATAGCTCGTTGATTTTGAA |
| Env4-S | ATTACACAGGCACAGGACCAA |
| Env4-R | GGCCAGCAGATAATCCAGAG |
| Env5-S | GGAGCAGTAGGAGTGGGGAC |
| Env5-R | ATCCTTCCTGTACTTTGGCTAT |
| Env6-S | TTTGAAGGCTTAGTAGGGGG |
| Env6-R | ATCTAACCCTCGCCTATTCT |
| Env7-S | CCCAGGCTACTCTATTATTCTACA |
| Env7-R | AAAGCAGTTACAGAGGTTTCTATT |
| Env8-S | TAGCTGCYAACCAAAGAATAGA |
| Env8-R | ATCATTAAATCYRACAGAGGTTAT |
| Env9-S | CCTCTTTTGTTTTATTACCTGCTA |
| Env9-R | GCAGAGCCTTGGAAGTATTT |
| RPL19-F | GAAGGTCTGGTTGGACCCCA |
| RPL19-R | GTATTTTTCCGGCATCGAG |
| 5′ RACE | |
| Race R | TTCCCCCCTTTAATATTGTGCTC |
| 3′ RACE | |
| Race F | TTTATAGGTTTACTCGGGTTTGTTTG |
| In situ hybridization probe synthesis | |
| Syncytin-Mar1-ISH-F1 | ATTTAGTTTGGTTTCCTTTGA |
| Syncytin-Mar1-ISH-R1 | CCACATGAGTCTTATTCCACA |
| Syncytin-Mar1-ISH-F2 | ACTTGGTAAGGAATGTATTGG |
| Syncytin-Mar1-ISH-R2 | CTAGATCAAGTCCTTTCCCAA |
| Syncytin-Mar1-ISH-F3 | GTTGGGGCTATAGAACTCCTA |
| Syncytin-Mar1-ISH-R3 | TTCAATAGCTGCTTCCATAGT |
| Amplification of genomic syncytin-Mar1 complete sequence | |
| Forward primer | TAGGTTTACTCGGGTTTGTTTG |
| Reverse primer downstream of the env (M.sibirica) | TTGTGGGCTGAGGATATAGTTC |
| Reverse primer downstream of the env (Tamias) | CCCCACAATTTAATCTTTTAC |
| Reverse primer downstream of the 3′LTR | GTTCCAGAATAGGCAGACAA |
| Amplification of genomic syncytin-Mar1 internal sequence | |
| Syncytin-Mar1-S | AGTGGAAATCATGTGAACCAT |
| Syncytin-Mar1-R | ACACACCCAACAGTTTTTAAC |