FIG 5.

Expression of gB in the corneas (A) and TG (B) of infected mice. CD8α^−/− and WT mice were immunized as described in the legend to Fig. 1 and ocularly infected as described in the legend to Fig. 2. gB expression in the cornea and TG was determined on days 3 and 5 p.i. In each experiment, an estimated relative copy number of HSV-1 gB was calculated using standard curves generated from pAC-gB1. Briefly, the DNA template was serially diluted 10-fold such that 5 μl contained from 10³ to 10¹¹ copies of LAT and then subjected to TaqMan PCR with the same set of primers. By comparing the normalized threshold cycle of each sample to the threshold cycle of the standard, the copy number for each reaction was determined. GAPDH expression was used to normalize the relative expression of each transcript in the cornea and TG of infected mice. Each bar represents the mean ± SEM from 6 corneas or TG.