FIG 9.

Expansion of CD11c⁺ CD4⁺ cells after injection of mice with GM-CSF. WT mice were injected once with GM-CSF DNA or Flt3L DNA or mock injected, as described in Materials and Methods. At 2 weeks postinjection and prior to HSV-1 infection, the spleens of some of the injected mice were harvested, and single cells were prepared, stained with anti-CD3, anti-CD4, anti-CD8α, and anti-CD11c MAbs, and analyzed by flow cytometry. CD3-negative/CD11c-positive cells were gated on expression of CD8α and CD4 cells. A minimum of 10⁴ events was acquired on a gate including viable cells. The mean percentages of CD3⁻ CD11c⁺ CD4⁻ CD8α⁺ or CD3⁻ CD11c⁺ CD4⁺ CD8α⁻ cells are shown for each treatment from two experiments.