FIG 7.

Downregulation of CD4 and MHC class I molecules by Nef. The CEM T1 permissive cell line was infected with VSV-G-pseudotyped recombinant reporter viruses, and levels of CD4 and HLA-A*02 were measured on all reporter-positive cells 3 days later. (A) Levels of Nef-mediated downregulation were compared to expression by a panel of control viruses, which included the NL4-3 Nef (“wild type”), ΔNef, Nef LL→AA (deficient only in CD4 downregulation), and Nef M20A (deficient only in MHC class I downregulation) viruses. (B) nef quasispecies from acutely infected subjects from the first and last study visits (designated “v1” for visit 1, etc.) were cloned and tested in bulk. Representative dot plots for subjects 01 and 02 are shown.