Skip to main content
. 2014 Jul;88(14):8139–8152. doi: 10.1128/JVI.00425-14

TABLE 5.

Immunogenicity and protective efficacy of H5N1 and H7N3 ca vaccines in AGMsa

Vaccine/no. of doses Challenge virus AGM no.b Serum Ab response
 Titer of challenge virus in respiratory tissues(log10 TCID50/g)
 Titer of challenge virus in swabs and lavage fluid(log10 TCID50/ml)
HAI
 MN
Day 42c Day of challenged Day 42e Day of challenge Nasal turbinates Trachea Lung Nasal/pharyngeal swab Tracheal lavage fluid
Mock/1 H5N1 wt H5-M 1 <20 <20 <20 <20 ≤1.5 4.2 6.6 ≤1.5 ≤1.5
H5-M 2 <20 <20 <20 <20 4.4 5.4 5.5 2.7 3.2
H5-M 3 <20 <20 <20 <20 3.4 6.7 5.8 4.2 ≤1.5
H5N1 ca/1 H5N1 wt H5-1D 1 <20 <20 <20 <20 ≤1.5 ≤1.5 4.4 ≤0.5 1.5
H5-1D 2 <20 <20 <20 <20 ≤1.5 3.7 7.1 3.5 3.7
H5-1D 3 <20 <20 <20 <20 2.5 4.2 4.3 ≤0.5 3.0
H5N1 ca/2 H5N1 wt H5-2D 1 1280 320 1810 905 ≤1.5 ≤1.5 ≤1.5 ≤0.5 ≤0.5
H5-2D 2 80 20 254 113 ≤1.5 ≤1.5 ≤1.5 ≤0.5 ≤0.5
H5-2D 3 640 160 1613 226 ≤1.5 ≤1.5 ≤1.5 ≤0.5 ≤0.5
Mock/1 H7N3 wt H7-M <20 <20 <20 <20 ≤1.5 5.7 4.2 4.0 4.4
H7N3 ca/1 H7N3 wt H7-1D 1 40 40 81 256 ≤1.5 ≤1.5 ≤1.5 ≤0.5 ≤0.5
H7-1D 2 20 20 102 128 ≤1.5 ≤1.5 ≤1.5 3.37 4.0
H7-1D 3 80 80 162 362 <1.5 ≤1.5 ≤1.5 ≤0.5 ≤0.5
a

ca, cold adapted.

b

AGM numbers are expressed as follows: subtype of vaccine (H5, H5N1 ca vaccine; H7, H7N3 ca vaccine)-number of doses (1D, one dose; 2D, two doses; M, mock [L15 medium]) followed by animal identification number.

c

The HAI assay was performed using 1% horse red blood cells, and titers are expressed as the highest dilution of serum that inhibited agglutination of 8 hemagglutination units (HAU) of virus. The lower limit of detection was 20. Antibody titers from individual animals are listed.

d

AGMs were challenged on day 59 with H5N1 wt virus and on day 63 with H7N3 wt virus. Serum samples from AGMs were collected before challenge infection. The lower limit of detection of virus in tissues was 1.5 log10 TCID50/g and in swabs and lavage fluid was 0.5 log10 TCID50/ml.

e

MN titers are expressed as the serum dilution that inhibited infectivity of 100 TCID50 of virus in a microneutralization assay using MDCK cells. The lower limit of detection was 20. Antibody titers from individual animals are listed.