Table 3.

Details of the stereological procedure for determining total number of cells in the human cerebrum^* using the optical fractionator method.

Case no.	∑s	ssf−1	asf−1	h(μm)	t(μm)	hsf−1	∑CF	∑n	∑g	CE	Total glia	Total neuron	Total no. cells
1	14	0.003	2 × 10−5	15	44.9	0.33	563	272	877	0.05	77 × 109	24 × 109	101 × 109
2	20	0.005	2 × 10−5	15	39.2	0.38	409	325	1274	0.04	65 × 109	17 × 109	82 × 109
3	19	0.005	2 × 10−5	15	39.7	0.38	777	347	1282	0.04	67 × 109	18 × 109	85 × 109
4	20	0.005	2 × 10−5	15	37.9	0.39	663	325	1353	0.05	67 × 109	16 × 109	83 × 109
Mean	18	0.0045	2 × 10−5	15	40.4	0.37	603	317	1197	0.045	69 × 109	19 × 109	88 × 109

ssf-1, reciprocal value of the section sampling fraction; asf-1, reciprocal value of the area sampling fraction; h, height of the counting frame; t, section thickness; hsf1, reciprocal value of the thickness sampling fraction; ∑ CF, number of unbiased counting frames used; ∑ n, number of neurons counted; ∑ g, number of glia cells counted; CE, coefficients of error of the estimates as in Gundersen et al. (1999).

^*Cerebrum includes the whole hemisphere, e.g., cortex, white matter, and central gray.