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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biotechnol Bioeng. 2013 Sep 13;111(1):1–15. doi: 10.1002/bit.25096

Figure 5.

Figure 5

Evolution of Cre specificity. A: loxP target. Base positions altered for selection with randomly mutated Cre variants highlighted orange (Buchholz and Stewart, 2001), those positions altered for selection with Cre variants that contain targeted substitutions highlighted red (Santoro and Schultz, 2002). Black arrows indicate the location of the scissile phosphates (B). Top and bottom: Cre monomer (light orange cartoon) in complex with loxP (blue and purple surface). Mutations identified by (top) substrate-linked protein evolution (SLiPE) and (bottom) those positions targeted for randomization for FACS selection shown as red sticks. Top and bottom: Substituted positions within each loxP target denoted purple. PDB ID is 1CRX (Guo et al., 1997).