Figure 10.

Immune responses to Theiler's murine encephalomyelitis virus (TMEV) during the acute stage of infection. A. Mononuclear cells (MNCs) were isolated from spleens of induced regulatory T cell (iTreg)‐early mice (open bars) or control mice (closed bars) on the peak of the acute stage and stimulated with TMEV at a multiplicity of infection (MOI) of 5, 1, 0.1 or by irradiated TMEV‐infected spleen cells that were incubated with TMEV overnight to act as APCs. Lymphoproliferation was quantified by a [³ H]thymidine incorporation assay. The two groups of mice had similar levels of TMEV‐specific lymphoproliferation (at 1 MOI, P = 0.98). Stimulation index = [counts per minute (cpm) of treatment group]/(cpm of no treatment group). Results are mean + standard error of the mean (SEM) from three pools of spleens from two mice per pool. B. The levels of total IgG against TMEV in sera were titrated by enzyme‐linked immunosorbent assays. The two groups of mice had similar anti‐TMEV antibody responses (P = 0.37). Values are mean absorbance (Abs) + SEM of six sera per group.