Figure 11.

Cytokine responses to Theiler's murine encephalomyelitis virus (TMEV) during the acute stage of infection. Mononuclear cells (MNCs) from the induced Treg (iTreg)‐early mice (open bars) produced more anti‐inflammatory cytokine interleukin (IL)‐10 (A) and IL‐4 (B) and less inflammatory cytokine interferon (IFN)‐γ (C) and IL‐17 (D) in response to concanavalin A (ConA) or TMEV compared with control mice (closed bars). MNCs were isolated from the spleens of iTreg‐early or control mice and stimulated with ConA or TMEV for 48 h. The levels of cytokine production in the culture supernatants were measured by enzyme‐linked immunosorbent assays. Results are representative of mean + standard error of the mean from two to three pools of spleens with two spleens per pool from four experiments. Abbreviation: ND = not detectable.