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. 2014 May 30;155(8):3065–3078. doi: 10.1210/en.2013-1916

Figure 3.

Figure 3.

The Kiss1/Kiss1r signaling system is expressed in the ovaries, oviducts, and uteri of Kiss1−/− and WT mice. In the ovaries, oviducts, and uteri of E2/gonadotropin-primed/superovulated KO (Kiss1−/−) female mice, relative to the expression of two housekeeping genes (Hprt, Sdha), the expression of Kiss1 and Kiss1r (A and C) [for WT, n = 6; for KO (Kiss1−/−), n = 4] was determined by quantitative RT-PCR. The expression of Kiss1r was further determined by in situ analysis (D, arrowheads show Kiss1r is localized to the luminal epithelium) [for WT, n = 4; for KO (Kiss1−/−), n = 4], whereas the expression of Kiss1 was determined by immunofluorescence (B) [for WT, n = 4; for KO (Kiss1−/−), n = 4]. Error bars represent SEM. DIC, differential interference contrast; GE, glandular epithelium; KO, knockout; L, lumen; LE, luminal epithelium; SL, subluminal epithelial layer; St, stroma; HOECHST, nucleic acid stain that identifies nuclei.