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. 2014 May 5;155(8):3036–3046. doi: 10.1210/en.2014-1094

Figure 5.

Figure 5.

Incubation of gastrocnemius muscles with insulin (10 mU/mL) for 30 minutes increases the phosphorylation of Akt Ser473 in both control and LP offspring. A, Representative blots showing bands for phospho-Akt Ser473 and total Akt levels along with GAPDH. B and C, Densitometric analyses of Akt Ser473 (B) and total Akt (C) levels in control and LP rat tissues treated with vehicle and insulin. Values were normalized with the loading control, GAPDH. Results are presented as means ± SEM (n = 4–5/group) *, P < .05).