Figure 5.

Effects of OT on TH-EGFP neurons in mice. Spontaneous EPSCs were recorded in voltage clamp. A, A comparison of the frequency of sEPSCs between a control period (aCSF) and a 5-minute bath application of OT (500 nM). Responders were neurons in which OT produced a significant increase in sEPSC frequency (P < .05; KS test: 8 of 17 neurons), and nonresponders were neurons in which OT had no significant increase in the sEPSC frequency (P > .05, KS test: 9 of 17 neurons). B, Frequency was compared between a control period (aCSF) and a 5-minute bath application of the antagonist, L-371,257 (500 nM) alone and coapplication of L-371,257 with OT (n = 12). Amplitude (picoamperes [pA]) (C) and decay time (milliseconds) (D) were also measured. Data are expressed as mean ± SEM. Group differences were made with respect to treatments. *, P < .05 (one-way ANOVA).