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E2 activates mitochondrial eNOS in HUVECs. HUVECs were treated with or without 10 nM E2β for different times. Activation of eNOS in the purified mitochondria and whole-cell extracts was measured by immunoblotting with specific antibody against ser1177 phosphorylated eNOS. Images (a) show blots of a typical experiment and graph (b) summarized data (mean ± SEM) from 3 independent experiments. *, P < .05.
