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. 2014 Jul 1;17(7):742–748. doi: 10.1089/jmf.2013.2978

FIG. 6.

FIG. 6.

Effect of Pro C1 on cGMP level in 1.0 μM PE-stimulated intact thoracic aortic rings. Pro C1 (50 μg/mL) was added to 1.0 μM PE-stimulated thoracic aortic rings (about 20 mg). (A) The cGMP level (pmol/mg protein) was determined by a cGMP enzyme immunoassay. The results are expressed as mean±SEM (n=4). *P<.05 was considered to be significant. 100 μM ACh was used as a positive control to confirm the vascular tone of the thoracic aortic rings in this experiment. (B) Proposed mechanism(s) of Pro C1-induced vasorelaxation. ACh, acetylcholine; cGMP, cyclic guanosine monophosphate.