Table 1.
Oligonucleotides Used in This Study
Genomic target | Oligonucleotide name | Forward (5′-3′) | Oligonucleotide name | Reverse (5′-3′) | Reference |
---|---|---|---|---|---|
Hantaviruses S-segment | DOBV-M6 | AGYCCWGTNATGRGWGTRATTGG | DOBV-M8 | GAKGCCATRATNGTRTTYCKCATRTCCTG | Essbauer et al. 2006 |
PUU Fpuni | TAGTAGACTCCTTGAARAGCTRCTACGA | cPUUV1122 | GCCATDATDGTTTYCTCAT | Mertens et al. 2011 | |
PUU M6 | AGYCCWGTNATGGGDGTNATTGG | cPUUV 1910 | TAGTAGTAKVCTCCTTGAAA | Essbauer et al. 2006 | |
DOBV Fpuni | TAGTAGTAKRCTCCCTAAARAG | cDOB_S_ FP | CACGGGAGGTCAAGCCA | This paper | |
DOBV_S_957F | TCTGGGTCTTTGCAGGAGCA | cDOBV_S_1620R | AGGTAGTGTTGTTGTTTTGAGGTA | This paper | |
Hantaviruses M-segment | PUU B3 | CARTTACARAAYCCIGCMAATGA | PUU C2 | CCAACTCCTGAACCCCATGC | Asikainen et al. 2000 |
DOBV_M_kroa1 | Available upon request | DOBV_M_kroa2 | Available upon request | Plyusnina et al. 2011 | |
Rickettsia spp. | PanRick_2_for | ATAGGACAACCGTTTATTT | PanRick_2_rev | CAAACATCATATGCAGAAA | Wölfel et al. 2008, Schex et al. 2011 |
Probe: PanRick_3_taq | FAM-CCTGATAATTCGTTAGATTTTACCG-TMR | ||||
ompB -120-2788 | AAACAATAATCAAGGTACTGT | ompB-120-3599 | TACTTCCGGTTACAGCAAAGT | Roux and Raoult 2000 | |
TBEV | F-TBE1 | GGGCGGTTCTTGTTCTCC | R-TBE1 | ACACATCACCTCCTTGTCAGACT | Schwaiger and Cassinotti 2003 |
Probe: TBE-WT | FAM-TGAGCCACCATCACCCAGACACA-TMR | ||||
mt-Cytb | L14648 | TGAATYTGAGGRGGATTCTCAGTA | H16 | CWGGTTGRCCTCCRATTCAWGT | Essbauer et al. 2006 |
CB_F | ATGACARTCATCCGAAARAAACAC | CB_R | TTARTCTAGGTCYAKRATGTYGTTTTC | Dekonenko et al. 2003 |
TBEV, tick-borne encephalitis virus; mt-Cytb, mitochondrial cytochrome b gene.