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. Author manuscript; available in PMC: 2015 Feb 10.
Published in final edited form as: Nature. 2014 Jul 10;511(7508):184–190. doi: 10.1038/nature13323

Figure 4. LPS tolerance induces AhR- and Src-dependent IDO1 phosphorylation.

Figure 4

a, Phosphorylation of IDO1 in total splenocytes isolated from LPS-tolerant mice in one experiment of three. Ratios are mean values from the three experiments. *P < 0.001 (15’ vs. single LPS exposure; two-tailed Student’s t-test). b, Splenic immunofluorescent staining. Scale bar, 100 μm. c, Phosphorylation of IDO1’s ITIM2 in WT and AhR-deficient cDCs. One experiment of three, ratios being means from the three experiments. *P < 0.001 (30’ vs. single LPS exposure; two-tailed Student’s t-test). d, Immunoblot analysis of Src and phosphorylated Src-Y416 (pSrc) in WT and AhR-deficient cDCs in one experiment of three. Ratios are means of the three experiments. *P < 0.001 (5’ and 15’ vs. single LPS exposure; two-tailed Student’s t-test). e, Production of TGF-β by Src-competent cDCs in response to single or double LPS exposure. Means ± s.d. from three experiments; **P < 0.001 (two-tailed Student’s t-test).