Figure 3.

Transcriptional activity of prostacyclin synthase (PGIS) promoter single-nucleotide polymorphism (SNP) 4 C325A variants. The transcriptional activity of the PGIS promoter SNP4 C325A variants was determined using the Luciferase reporter normalized to cotransfected Renilla reporter activity. Results from two different cell types in addition to normal are shown: immortalized human bronchial epithelial cell line (BEAS-2B) and human umbilical artery endothelial cells (HUAEC). The SNP4 C325A variants (C major allele, A minor allele) were tested in the context of the G22_G45_5wt PGIS promoter haplotype where the SNP4 A allele is most commonly associated. The data were normalized to the activity of the most common PGIS promoter allele (T22_G45_6wt_C325). Error bars are the standard error of the mean and significance (**P = 0.005–0.05) was determined by two-tailed t tests.