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Protein expression profiling of primary human hepatocytes and HepG2 cells. Total cellular protein was isolated from primary human hepatocyte cultures and cultures of HepG2 cells, and equal quantities of protein from the respective samples were applied to 10% denaturing gels, separated by SDS-PAGE and then transferred to PVDF membranes and assessed by immunoblotting analyses, as described in “Materials and Methods” section.
