Fig. 1.

Pharmacological activation of PKC with PMA leads to inhibition of spontaneous transient large-conductance voltage- and Ca²⁺-activated K⁺ (BK) currents (TBKCs) in guinea pig detrusor smooth muscle (DSM) cells. A: original recording illustrating the inhibitory effect of PMA (100 nM) on TBKC activity in a guinea pig DSM cell. B: summary data depicting the inhibitory effect of 100 nM PMA on amplitude and frequency of TBKCs (n = 7, N = 7). **P < 0.01, ***P < 0.001. C: original recording illustrating lack of an effect of 100 nM 4α-PMA on TBKC amplitude and frequency in a DSM cell. D: summary data showing lack of a significant effect of 4α-PMA (100 nM) on amplitude and frequency of TBKCs (n = 7, N = 5, P > 0.05).