Fig. 3.

PKC activation with PMA has no effect on voltage-step-induced whole cell steady-state BK currents after inhibition of all known major sources of Ca²⁺ for BK channel activation. A: original recording illustrating voltage-step-induced whole cell steady-state BK current prior to (control) and after addition of 100 nM PMA. B: current-voltage relationship illustrating lack of an effect of PMA (100 nM) on voltage-step-induced whole cell steady-state BK current in DSM cells (n = 6, N = 4, P > 0.05). All recordings were performed with thapsigargin (100 nM), ryanodine (30 μM), and nifedipine (1 μM) in the bath solution.