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Heparin-mediated immobilization of TFPI-1 promotes cell adhesion. Wells were coated with 5 U/mL heparin followed by blocking with BSA. The indicated concentrations of TFPI-1 were either added together with the cell suspension or preincubated with the heparin-coated well for 1 hour, followed by removal of unbound inhibitor before the addition of the cell suspension. The cell adhesion buffer contained 10 nM VIIa, and adhesion was determined after 1 hour.
