Figure 7.

Cooperation of TF with integrins in the migration of J82 bladder carcinoma cells. The lower side of the migration filter was coated with the indicated concentration of fibronectin (FN) followed by a second coating with anti-TF antibody TF9-6B4 (50 μg/mL) or TFPI (1 μg/mL), as indicated. J82 cell migration was quantified after 5 hours. Catalytically inactive VIIa Ala 344 was present at 10 nM in the upper and lower migration chamber, where indicated. (a) Enhanced migration on fibronectin/anti–TF-coated filters is blocked with soluble TF (100 μg/mL) or the inhibitory anti-β₁ integrin antibody AIIB2 (50 μg/mL), both present in the upper and lower compartments of the migration chamber. (b) VIIa is required for enhanced migration on TFPI/fibronectin-coated surfaces. (c) TFPI/VIIa enhanced migration depends on the amount of immobilized fibronectin.