Figure 4.
PY-associated PI 3-kinase activity. Tyrosine-phosphorylated proteins were immunoprecipitated (immpt.)using an anti-PY antibody (PY 99). PI 3-kinase activity was assayed in vitro using PI as substrate. Top panel shows a phosphorimage of a representative TLC plate. Quantified data from multiple experiments are given in the graph below. Data are means ± SEM. n = 6–8 per group. *P < 0.001 compared with Rest. #P < 0.005 compared with Insulin.