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GSK-3 Ser21 phosphorylation. GSK-3 Ser21 phosphorylation was identified by immunoblotting using a phospho-specific antibody after SDS-PAGE. Top panel shows a representative blot, and the quantified data from multiple experiments are given in the graph below. Data are means ± SEM. n = 6–8 per group. *P < 0.004 compared with Rest. #P = 0.05. ‡P = 0.08 compared with Insulin.
