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. 1999 Nov 15;104(10):1375–1382. doi: 10.1172/JCI6097

Figure 1.

Figure 1

Human tracheal aspirates from asthmatics induce MUC5AC in airway epithelial cells in culture. (a) NCI-H292 cells were treated for 8 hours with aspirates from 5 healthy control (Normal) and 5 asthmatic human subjects. Cells used as negative (–) controls were in SFM and cells used as positive (+) controls were exposed to vanadate. RT-PCR was performed using primers for MUC5AC and GAPDH. (b) NCI-H292 cells were transfected with the 3.7-kb MUC5AC luciferase construct and challenged with asthmatic or non-asthmatic tracheal aspirates for 16 hours. RLU, relative luciferase units. Bars represent means ± SEM (n = 5 patients/group in duplicate). *Significantly different from SFM control; P < 0.05.