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. 1999 Nov 15;104(10):1375–1382. doi: 10.1172/JCI6097

Figure 7.

Figure 7

The effect of recombinant human cytokines on MUC5AC. (a) NCI-H292 cells were transfected with the 3.7-kb MUC5AC luciferase construct and challenged with rhIL-9 (circles), rhIL-5 (squares), or rhIL-13 (triangles) for 14–16 hours. Bars represent means ± SEM (n = 3 wells in triplicate). (b) Steady-state MUC5AC and cyclophilin were measured by RPA in NCI-H292 cells treated with IL-9. (c) MUC5AC and GAPDH mRNA were also quantified by RT-PCR in human primary bronchial epithelial cells treated with rhIL-9.