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. 1999 Nov 15;104(10):1383–1391. doi: 10.1172/JCI7537

Figure 1.

Figure 1

RT-PCR of ECL-cell and parietal-cell RNA. (a) RT-PCR performed on ECL cell RNA using primers specific to the third intracellular loop splice variants. Sense primers (S1, HipS, HOPS, SV3AS) were used together with antisense primers (S1, HipS, HOPS, SV3AS) that spanned the intron-exon junctions as described previously (23). Amplification using primers for β-actin was performed as a control and is shown in the last lane. (b) RT-PCR performed on purified ECL and parietal cells (PC). The primers used were designed to amplify PAC1, histidine decarboxylase (HDC), H,K-ATPase, and β-actin in these 2 purified cell preparations (>85%).