Figure 2.

PACAP-induced stimulation of Ca2⁺_i in ECL cells. (a) PACAP-induced intracellular signaling in isolated ECL cells: video imaging using Fura-2/AM–loaded ECL cells as detailed in text. PACAP-27 was administered at a dose of 1 nM; the induction of a typical biphasic Ca²⁺ release is shown. (b) Dose response to PACAP of the averaged [Ca²⁺]_i stimulation in response to various concentrations of PACAP-38. Data are presented as the Ca²⁺ response index, calculated as: (peak [Ca²⁺]_i of responding cells × the percentage of cells responding)/100.