Skip to main content
. 1999 Nov 15;104(10):1449–1457. doi: 10.1172/JCI7936

Figure 1.

Figure 1

ADS delivers antigen to the EBV B–cell surface at high concentration. (a) The b-PWM has preferential affinity for disulfide-bonded Ig-like molecules such as surface Ig on the EBV B cell. The b-PWM is then bound to avidin, which can bind up to 3 molecules of biotinylated antigen. (b) The PWM-based ADS is completely internalized in 6 hours. Priess EBV B cells were incubated sequentially on ice with each component of the ADS (b-PWM, avidin-D, biotinylated antigen [TT]) and then incubated in culture medium at 37°C for 0, 1, 3, or 6 hours and removed for analysis of surface TT by flow cytometry, using rabbit anti-TT antiserum and FITC goat anti-rabbit Ig. Compared with background (solid line), surface expression was high at 0 hours (long dashes) but had diminished by 1 (dashes broken by dots) and 3 hours (short dashes). ADS-delivered antigen was completely internalized by 6 hours (dotted line).