Figure 2.

Expression of (a) DT-A, native endogenous trefoil peptides, and (b) the MUC2 and MUC3 genes in gastrointestinal tissues of mITF/DT-A transgenic mice and normal control mice. Northern blot analysis using 30 μg of total RNA from various tissues of both DT-A transgene–positive and –negative mice was performed as detailed in Methods; blots were hybridized under high-stringency conditions with ³²P-labeled probes made by the random-primed DNA labeling method. The membranes were exposed at –80°C overnight (ITF and GAPDH) or for 3 days (DT-A, MUC2, and MUC3). DT mRNA was detected strongly in the colon, weakly in the jejunum and ileum, and faintly in the duodenum, but not at all in stomach or kidney of mITF/DT-A transgenic mice, in a manner parallel to that of the ITF gene. Arrow indicates predominant DT transcript. No DT-A mRNA was detected in tissues of normal control mice.