Skip to main content
. 2013 Sep 17;2(9):e122. doi: 10.1038/mtna.2013.49

Figure 5.

Figure 5

Positive correlation of average vector copy number (VCN) per cell in transduced CD34+ cells with peripheral blood cells when using provirus-specific real-time PCR system. (a) We evaluated VCN in transduced CD34+ cells using the SIN-LTR–specific probe/primers, which resulted in positive correlation of VCN in transduced CD34+ cells with both granulocytes (P = 0.01) and lymphocytes (P < 0.01) when excluding outliers of three animals with high-marking levels (VCN >6). The transduced CD34+ cells had nine- to tenfold higher VCN, as compared with granulocytes and lymphocytes. (b) We summarized comparison data between in vitro and in vivo, when using SIN-LTR probe/primers. *Twofold scores were used in competitive assay; **Excluding in vivo VCN >6. GFP, green fluorescent protein; SIN-LTR, self-inactivating long terminal repeat; YFP, yellow fluorescent protein.