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. 2014 May 16;2(5):e00284. doi: 10.14814/phy2.284

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Stress‐induced effects on glucose and NEFA circulating levels. Plasma from stressed and control rats was isolated from whole blood after centrifugation. (A) Chronic stress is associated with increased circulating glucose levels. (D) NEFA plasma levels are elevated in stressed rats compared to controls. At the conclusion of the 35‐day stress protocol, rats were restrained and blood was collected from their tails at the described intervals and measured using an AccuCheck glucose counter. (B) Stressed rats show reduced ability to remove glucose from the circulation after a 2‐hr challenge (glucose 1 g/kg). Circulating glucose levels remain high 60 min after challenge in the stressed group (grey line). (C) Circulating insulin levels decreased with stressed compared to control rats. Data are presented as means ± SEM (Mann–Whitney, One way ANOVA, *P <0.05, **P <0.01, n =12 and n =6 for [D]).