Figure 5.

Effect of syntrophin γ2 on NBCn1 conductance. (A) Representative current–voltage (I–V) relationships in a syntrophin γ2‐expressing oocyte or an uninjected oocyte. Oocytes were clamped at −60 mV and subjected to the voltage command stepping from −140 to +40 mV. Steady‐state currents were recorded (n =5 for each). Recordings were done in HCO₃⁻/CO₂‐free ND96 solution. (B) I–V relationships in oocytes expressing NBCn1 and different amounts of syntrophin γ2. Large inward currents at negative potentials and outward currents at positive potentials are hallmarks for NBCn1 conductance. (C) Mean slope conductance. Slopes were determined near zero‐current voltages in I–V plots. Data were averaged from uninjected control oocytes (n =12), oocytes expressing syntrophin γ2 (n =16), NBCn1 (n =15), NBCn1 plus syntrophin γ2 at 2 ng (n =17), 10 ng (n =15), and 20 ng (n =24). n.s, not significant. P‐values were calculated to compare each NBCn1/syntrophin with NBCn1 only.