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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Thromb Haemost. 2014 May;12(5):680–689. doi: 10.1111/jth.12534

Figure 1. Purified fibrinogen contains all three chains (Aα, Bβ, and γA and/or γ’) at the expected molecular weights and is equally cleaved by human and mouse thrombin.

Figure 1

Unfractionated (UF), or purified γA/γA, or γA/γ’ fibrinogen were reduced and separated by 10% SDS-PAGE and detected by: A) Coomassie Brilliant Blue staining, B) polyclonal anti-fibrin(ogen) antibody, or C) 2.G2.H9 antibody against the γ’ chain. D) Purified human γA/γA (squares) or γA/γ’ (diamonds) fibrinogen was clotted in the presence of CaCl2 and human (closed symbols) or murine (open symbols) thrombin. Data show mean±SD, for experiments with human (n=3) and mouse (n=2) thrombin.